The 2Brothers project is a versatile microscopy imaging platform. The system is designed to be modular, allowing rapid switching between two main operating modes to accommodate a range of experimental needs.

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(Right) Low-magnification imaging (up to 10×) with moderate acquisition rates able to operate in both transmitted and reflected-light configuration. This mode utilizes an sCMOS camera (Quantalux® 2.1 MP), which enables image acquisition with relatively low read noise.

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(Left) High-magnification imaging (up to 40× using a Nikon Plan Fluorite objective) with Epi-fluorescence capability. This mode utilizes a CMOS camera (Kiralux® 1.3 MP), which can acquire full-frame images at up to 165 frames per second. The illumination path includes an integrated filter cube slot for rapid insertion of filters for fluorescence microscopy. This configuration allows the setup to operate as an Epi-fluorescence and transmitted light microscopy. 

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